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KMID : 0380020200350010057
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Korean Journal of Biotechnology and Bioengineering
2020 Volume.35 No. 1 p.57 ~ p.63
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Inhibitory Effects of the Halophyte Ligustrum Japonicum on Matrix Metalloproteinases Activity in PMA-stimulated HT-1080 cells
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Kim Jun-Se
Kong Chang-suk
Moon Ki-Hwan
Seo Young-Wan
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Abstract
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Matrix metalloproteinases (MMPs) are the zincdependent endopeptidases that cause the degradation and remodeling of extracellular matrix. They are associated with various physiological and pathological processes. Among the MMPs, gelatinases, MMP-2 and MMP-9, play a major role in the metastasis and invasion of cancer or tumor cells. In this study, the inhibitory effect of the halophyte Ligustrum Japonicum on MMP-2 and MMP-9 activity in phorbol-12-myristate-13-acetate (PMA)-stimulated HT-1080 cells was estimated by using enzyme-linked immunosorbent assay (ELISA), gelatin zymography, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay. Also, the effect of L. japonicum on HT-1080 cell migration was investigated by using wound-healing assay. The dried L. japonicum was extracted with dichloromethane (CH2Cl2) and methanol (MeOH), and the combined crude extracts were fractionated according to the solvent polarity to obtain the n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH) and water fractions. Of the fractions, 85% aq.MeOH fraction most effectively suppressed the enzymatic activity, cell mobility, and mRNA and protein expression of MMP-2 and MMP-9. Therefore, L. japonicum will be used as a valuable source of potential anti-invasive agents.
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KEYWORD
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Ligustrum japoinicum, matrix metalloprotienase, anti-invasive, HT-1080
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